DIY Glycolysis

Go on to the next step.

Step Six - Coupled Oxidation & Phosphorylation of Glyceraldehyde 3-phosphate

Product 1,3-bisphosphoglycerate

Well done. This is the key reaction of glycolysis. You've managed to put a high energy phosphate group onto your monosacharide without spending ATP. However, you did need to use an oxidising agent, NAD+, that will need to be recycled later, and so a reduction will be required at some point.

So far two ATP spent & subtrate oxidised by four electrons (Two electron oxidation for each molecule of substrate).